AN.005.R63
The rapid quench flow technique provides a powerful method of studying rapid reactions involving solutions that are not chromophores and so cannot be monitored by absorbance or fluorescence spectrophotometry, or are at concentrations too low to allow the use of an optical probe. This is illustrated by studies of the cleavage of the plasmid pAT153, a covalently closed circle of DNA, by the restriction enzyme EcoRV.