AN.008.S20
The use of a commercial spectrophotometer equipped with a diode array detector to study rapid reaction kinetics has great potential due to the simultaneous acquisition of multi-wavelength data. This is made possible by the addition of an optimised stopped-flow accessory. The reaction of Ellman’s reagent with thioglycerol is a well characterised coupled two step process and is used to illustrate how this combination can be used to acquire complex data which can then be processed using multi-variate global analysis methods.