AN.011.S20
Measurement of changes in concentration of intracellular cations such as Ca²+ is important in understanding the mechanisms of many cellular processes. The use of a fluorescence indicator in combination with fluorescence spectrophotometry is a well established technique for making these measurements. The rate of displacement of the fluorescence indicator from the bound complex is important to ensure the validity of quantitative results. The rate of this displacement can be measured using a commercial spectrofluorimeter equipped with a stopped-flow accessory.