AN.015.S20
The hydrolysis of 2,4-Dinitrophenol Acetate by Sodium Hydroxide has become a standard reaction for testing the performance of fast kinetic instruments. The reaction was first published by H Gutfreund (Methods in Enzymology 16 (1969) pp 229-249) and can be measured with a Varian Cary 50 Spectrophotometer with the SFA-20/Varian stopped-flow accessory because of the very fast time-base this spectrophotometer has.