AN.017.S20
The mechanism of the ATPase reaction of myosin and its subfragment, S1 has been studied with transient kinetic methods and is widely reported. Here, a derivative of ADP, mantADP [2′-3′-O-(N’-methylanthraniloyl-5′-O-diphosphate] in premixed complex has been displaced with excess ATP (Woodward, Eccleston and Geeves, 1991). This demonstrates the use of the SFA-20/Varian Stopped-Flow Accessory in a commercial fluorescence instrument.